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<p><b>BACKGROUND</b>A practical problem impeding clinical translation is the limited bone formation seen in artificial bone grafts. Low-pressure/vacuum seeding and dynamic culturing in bioreactors have led to a greater penetration into the scaffolds, enhanced production of bone marrow cells, and improved tissue-engineered bone formation. The goal of this study was to promote more extensive bone formation in the composites of porous ceramics and bone marrow stromal cells (BMSCs).</p><p><b>METHODS</b>BMSCs/β-tricalcium phosphate (β-TCP) composites were subcultured for 2 weeks and then subcutaneously implanted into syngeneic rats that were split into a low-intensity pulsed ultrasound (LIPUS) treatment group and a control group. These implants were harvested at 5, 10, 25, and 50 days after implantation. The samples were then biomechanically tested and analyzed for alkaline phosphate (ALP) activity and osteocalcin (OCN) content and were also observed by light microscopy.</p><p><b>RESULTS</b>The levels of ALP activity and OCN content in the composites were significantly higher in the LIPUS group than in the control group. Histomorphometric analysis revealed a greater degree of soft tissue repair, increased blood flow, better angiogenesis, and more extensive bone formation in the LIPUS groups than in the controls. No significant difference in the compressive strength was found between the two groups.</p><p><b>CONCLUSION</b>LIPUS treatment appears to enhance bone formation and angiogenesis in the BMSCs/β-TCP composites.</p>
Subject(s)
Animals , Male , Rats , Bone Marrow Cells , Physiology , Bone Transplantation , Calcium Phosphates , Pharmacology , Osteogenesis , Physiology , Stromal Cells , Tissue Engineering , Methods , Transplantation, Isogeneic , Ultrasonics , MethodsABSTRACT
To study the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, on the ratio of CD4(+)CD25(+) regulatory T cells and expression of transcription factor Foxp3 in mice with Lewis lung cancer.
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To study the effect of Feiyanning Decoction (FYN), a compound traditional Chinese medicine, on expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activated by tumor necrosis factor-α (TNF-α) in human lung adenocarcinoma epithelial cell line (A549).
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Malignant tumor cells were found with an abnormal cell cycle. Previous in vivo experiment had confirmed the Feiyanning's intervention effect on checkpoint signaling of G1/S in the cell cycle. This study was to further observe the expressions of nucleosome conformation-regulating factors intervened by Feiyanning decoction in S phase.
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Pain is one of the common symptoms of cancer which seriously affects the quality of life of the patients. Cancer pain is mainly treated with the three-step method, biological therapy or nerve block therapy based on antitumor therapy. However, up to 50 percent of patients with cancer-related pain do not receive adequate pain relief, affecting their physical and psychological well-being, and leading to a lower quality of life for the patient after conventional treatment. Clinical observation suggests that traditional Chinese medicine may alleviate cancer-related pain either by oral administration, topical administration, acupuncture or other means with continuing non-addictive and non-drug-resistant qualities. However, scientific evaluation of the efficacy of herbs in the treatment of pain is insufficient; the underlying mechanisms are unclear and, safety and toxicity remain a concern.
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Foxp3 is a novel member of forkhead and winged-helix transcription factor family.Foxp3 is specifically expressed in CD4+ CD25 + regulatory T cells,and Foxp3 is a critical regulator of CD4+ CD25 + regulatory T cell development and function maintain.With the development of molecular biology techniques,the understanding of Foxp3's structure and function is increasingly clear.Foxp3 plays an important role in the lung cancer's development,clinical treatment and prognosis.Thus to clarify the regulatory mechanism of Foxp3 expression is beneficial for the explanation of lung cancer's immunal environment and the establishment of a better immunal therapeutic regimen,so as to provide profound significance for lung cancer's prevention and treatment.
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@# ObjectiveTo evaluate whether β-tricalcium phosphate (β-TCP) combined with bone marrow mesenchymal stem cells (BMSCs) can be used for lumbar posterolateral spine fusion (PLF) instead of autogenous bone graft. Methods6 crab-eating macaques underwent bilateral PLF at L4-5, and divided into 3 groups that implanted β-TCP/BMSCs composite, autogenous bone, and β-TCP. Monkeys were sacrificed 12 weeks after implantation. Manual palpation, micro computed tomography, peripheral quantitative computed tomography (pQCT), and histology were used to assess bone formation. ResultsManual palpation showed that 75% of β-TCP/BMSCs composite group and autogenous group achieved solid spine fusion, whereas none of β-TCP group fused. Histological analysis showed that all of the β-TCP/BMSCs group achieved massive bone formation. Bone mineral density (BMD) evaluated with pQCT in the β-TCP/BMSCs group increased by additional new bone. Conclusionβ-TCP/BMSCs composite can be used for PLF instead of autogenous bone graft.
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To study the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, on gene expression of nuclear factor-kappaB (NF-kappaB) activated by tumor necrosis factor-alpha (TNF-alpha) in lung adenocarcinoma cell line (A549).
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To study the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, on proliferation of lung adenocarcinoma cell line A549 cells and their production of interleukin-6 (IL-6) and IL-8 induced by tumor necrosis factor-alpha (TNF-alpha).
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To study the apoptosis of human hepatocellular carcinoma cell line HepG 2 induced by pitavastatin (NK-104) and to investigate the change of caspase-3 activity.
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Objective To explore the capsular changes at the weight- bearing area of hip with osteoarthritis and its correlation to postoperative clinical results of Chiari pelvic osteotomy. Methods According to the Bombelli radiological classification, the 66 hips with osteoarthritis were classified into three groups: hypertrophic, normotrophic and atrophic. Specimens of the capsule at weight- bearing area were obtained during total hip replacement for osteoarthritis, and stained with HE, Van- Gieson and Safranin O. Histopathological changes of the capsules were observed and compared among the three groups. Results The hypertrophic group showed a high incidence of metaplastic cartilage (63.3％ ). Normotrophic group(32.1％ )and atrophic group(25.0％ ) showed a low incidence of metaplastic cartilage. The atrophic group had a higher incidence of fibrous synovitis or fibrosis(81.3％ ) than hypertrophic. Among the three group, the frequency of infiltration granulation tissues was low, the frequency of both infiltration of inflammatory cells and degeneration of collagen fiber was high. Conclusion Associating with clinical research, these findings suggest that the capsular changes at weight- bearing area are responsible for postoperative clinical results of Chiari pelvic osteotomy. Compared with normotrophic and atrophic type, the hypertrophic type has the best adaptation of Chiari pelvic osteotomy.
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50?10~(9)/L. Of the patients, 3 died of liver function exhaustion or disease relapse 1-12 months after transplantation , 2 are still alive with disease for 3-5 months , but others are still alive and disease-free for 3-64 months. Conclusions:PBSCT is a safe therapeutic option that can significantly improve in acute leukemia and malignant solid tumors.
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Background and purpose:A causal relationship between inflammation and cancer has been extensively investigated. Nuclear factor-kappa B (NK-?B) is a key regulator in inflammation-associated cancer, and is activated by a variety of stimuli. This paper studied the anti-inflammatory effect of NK-104 on NF-?B activated by TNF-? in hepatocellular carcinoma cell line (Huh 7). Methods:The cell proliferation was examined by the WST-8 assay. The NF-?B and I?B? expressions were evaluated by western blotting. IL-8 and its product were quantitated by ELISA.Results:TNF-?(1 ng/ml) strongly induced the expression of NF-?B by approximately 1.8-fold compared with the control in the nuclei of Huh 7 cells, the induced NF-?B expression was significantly suppressed by the addition of pitavastatin at 0.1 ?mol/L (P 0.05). Subsequently, the addition of TNF-? significantly increased IL-8 production, which could be reversed by the addition of NK-104.Conclusions:These results suggest that NK-104 at low dose (0.1 ?mol/L) inhibits NF-?B activation and decreases IL-8 production induced by TNF-?. It is therefore expected to play a role in the new strategy for the treatment of hepatocellular carcinoma.